Isolation of fungi using the diffusion chamber device FIND technology

• Benjamin Libor,
• Henrik Harms,
• Stefan Kehraus,
• Ekaterina Egereva,
• Max Crüsemann and
• Gabriele M. König

Beilstein J. Org. Chem. 2019, 15, 2191–2203, doi:10.3762/bjoc.15.216

• of them being the marine-adapted fungal strain Heydenia cf. alpina. The latter produced two new terpenoids, which are the first secondary metabolites from this genus. Keywords: FIND; fungal one-step isolation device; Heydenia cf. alpina; natural products; terpenes; Introduction Natural products

• litre of agar solution to obtain the required target concentration. Using this ratio, in our experiments, however, we had hardly any success (apart from one fungal isolate, i.e., Cladosporium allicinum and Heydenia cf. alpina from samples 2 and 3, respectively). Obviously in these experiments the

• salt tolerance or dependency. Thus, we analysed the growth behaviour of Cladosporium allicinum and Heydenia cf. alpina on agar plates containing growth medium of increasing salinity from 0–35‰ (sea water has a salinity of approx. 35‰). As outcome the diameter of radial growth was measured after 14 days